The cultivations in wood hot water extract (HWE) without autoclaving did not result in any substantial cellulose activity and this result was some what surprising, since it is not clear that the media contained compounds that inhibit Acetobacter xylinus 23769. This could have occurred due to contamination of the hot water extract, which was not sterilized. However,in the Uraki, Morito, Kishimoto, and Sano (2002) study, bacterial cellulose production was not observed in the unpurified water soluble fraction of waste liquor from atmospheric acetic acid pulping due to presence of antibacterial activity in the unpurified water soluble fraction . bacterial cellulose production was only observed after purifying the medium with activated carbon. There fore, wood hot water extract should also be studied in terms of antibacterial activity. Monosaccharide concentration also did not change significantly before and after autoclaving. Cultures at pH 5 and 26 and 30◦C and at pH 6 and 26◦C did not produce bacterial cellulose and the reason why bacterial cellulose is not produced at these conditions requires further investigation. The results in Table 2 indicate that glucose and xylose were the main nutrientsources in all cultivations.