Additional
sequences used in the analyses were downloaded from
Treefam [121]. Multiple alignments of predicted proteins
were generated with MUSCLE using the default settings
[122] and were subsequently inspected and corrected by
eye. Full alignments were trimmed using G-blocks [123]
and were run through ProtTest using the default settings
to determine the optimum evolutionary model for phylogenetic analyses [124]. Neighbor joining trees were constructed using MEGA [125] and maximum likelihood
analyses were conducted using PhyML [126] with the
amino acid substitution models specified from ProtTest.
Phylogenetic trees are available in Additional file 2.