In vitro kinetic studies on samples of Ls:AG-CH 1:10 and Ls:AG-CH 1:20 were performed, and the results are shown in Figure 6. The release profiles of the NPs indicate that the encapsulation of Ls was successful and the system can effectively sustain Ls release under the test conditions. Sustained release is important due to the increase in the availability, prolonging the larvicide effect. In the first five hours, in vitro release was similar for Ls:AG-CH 1:10 and Ls:AG-CH 1:20. However, after 24 h, it is observed that sample Ls:AG-CH 1:10 showed a slower release profile than Ls:AG-CH 1:20, resulting in 26.7% and 52.6% of the oil being released, respectively.