Fig. 1. Representative urogenital complexes (adrenal-kidney-gonad) of estrogen (E2) treated and control embryos at stage 26, incubated at male-producing temperature (MPT) or at female-producing temperature (FPT). (A) Urogenital complexes treated with the vehicle show well developed gonads. (B) In E2 treated embryos, the gonads are almost imperceptible (*). (C) At the scanning electron microscopy the gonad of control embryos at MPT is evident along the mesonephros. (D) Hypoplastic gonad from E2 treated embryo appears as irregular folds protruding on the mesonephros. (E) Higher magnification of the gonad shown in D. (F) The gonad of control embryos at FPT is shown. In all cases the gonad is marked by a dotted oval. Mes1⁄4mesonephros, G1⁄4gonad. Ad1⁄4adrenal. Scale Bars: A–B1⁄45 mm, C–D and F1⁄41 mm, E1⁄4500 μm