Plant material
The rose cultivars were brought from various
nurseries of Dehradun, Uttarakhand, India and
healthy rose plants were selected for tissue
culture studies. After cleaning and thorough
washing in running tap water for 5 minutes, the
nodal explants were treated with 0.1% (v/v)
Tween-20 for 15 minutes followed by
treatment in 0.2% (w/v) bavistin for 20
minutes and finally in 0.1% (w/v) mercuric
chloride for 2 minutes with each step followed
by washing in double distilled water several
times. Antioxidants treatment was given to
explants by using 0.2% (w/v) polyvinyl
pyrollidone (PVP) for 15 minutes.