Determination of sugar concentrations
in almond dusts
Sterile distilled water (19 mL) was added to triplicate 1-g samples
of almond dust in 50-mL conical centrifuge tubes to make
slurries. The tubes were then placed on a refrigerated Lab-Line
shaker (Barnstead Int., Dubuque, Iowa, U.S.A.) and rotated at 150
rpm for 24 ± 2 h at 4 ± 2 ◦C. Following agitation, each slurry was
filtered through 8 layers of sterile cheesecloth to obtain a supernatant,
which was stored for up to 24 h at 4 ± 2 ◦C until testing. Sucrose,
D-glucose, andD-fructose concentrations in the supernatant
were determined using an Enzymatic BioAnalysis kit (r-biopharm
GmbH, Darmstadt, Germany) according to the manufacturer’s instructions;
D-glucose was determined before and after the enzymatic
hydrolysis of sucrose, and then D-fructose was determined.