2.3. Data analysis
First, genotyping data were processed with the Excel Microsatellite Toolkit
[14] (http://www.animalgenomics.ucd.ie/sdepark/ms-toolkit/). Then, we used
the F-stat software (2.9.3.2) to determine the genetic variation within and
between breeds, the number of alleles, and the heterozygosities, and the Wright
F-statistics [25] and the DISPAN software [13] to calculate genetic distances
(DA) [12]. A principal components analysis (PCA) of allelic frequencies was
carried out according to the procedures described by Cavalli-Sforza et al. [4]
using SPSS (11.5.0). Phylogenetic analyses were conducted with the MEGA4
software package [22] using the neighbor-joining method [18].