Chlamydospores of P. ramorum isolate WSDA-1772 were
produced by a modified method described by Mitchell and
Kannwischer-Mitchell (1992) and stored in autoclaved sand at
4 C as described by Widmer et al. (1998). Five agar plugs
(5-mm-diameter) containing actively growing mycelium of
P. ramorum isolate WSDA-1772 were placed in 20 ml of 20% sterile,
clarified V8 broth in sterile Petri plates (100-mm-diameter) and
stored at 20 C in the dark. After 4 weeks, th