Cumulus-oocyte complexes (COCs) were aspirated from the ovaries of sexually mature, unsynchronized Bos indicus (Brahman) cows every 2 wk (n = 7 replicates), matured in vitro, and fertilized with either nonsorted frozen-thawed (control), SF, FS, or FSF sperm from three bulls of different breeds (Jersey (n = 3 ejaculates), Holstein (n = 2 ejaculates), and Simmental (n = 2 ejaculates)).