Artificial insemination (AI) is the first generation reproductive biotechnology that has made a profound contribution to the genetic improvement, particularly in dairy cattle. This impact would not have been possible without successful freezing of bull semen. The ideal semen analysis would be simple and effective, allowing the breeding capacity of a particular ejaculate to be predicted. A fertile ejaculate must meet certain semen parameter quality standards, such as: progressive motility, normal morphology, active energy metabolism, structural integrity and functionality of the membrane, penetration capacity and optimum transfer of genetic material. As most of bull semen were cryopreserved for future use. The process of cryopreservation represents an artificial interruption of the progress of the spermatozoon towards post-ejaculation maturation and fertilization