Banana is an important fruit crop of the family musaceae. In Kanyakumari District, many varieties of banana are cultivated. Matti is one among them which fetches good price in the market and farmers called, it as money spinner. This study carry out the micro propagation of banana cv. Matti. The sword suckers of three months old were used as explants. The explants sterilised thoroughly and MS media supplemented with the cytokinins like BAP, kinetin, zeatin and adenine sulphate in different concentration (0.5 to 5.5 mg/l) were used for the multiple shoot generation and shoot growth. Among the tested cytokinins BAP in medium concentration performed well and higher concentration of all tested cytokinins showed declining effect. Generated shoot initials were transferred to the rooting media after three sub cultures. For rooting MS media was supplemented with different levels of IBA. Rooting was highly promoted in the medium concentration of IBA. After 115th day of inoculation the plantlets were transferred to green house for hardening and subsequently planted in the field after 30 days of acclimatization.