Raw milk samples were taken from the University farm office of Walailak
University, Nakhon Si Thammarat in Thailand. Each sample was heated at 80 °C for
30 min in a water bath. Then the liquid was serially diluted in sterile distilled water, and
the dilution from 10-1 - 10-10 was plated on a nutrient agar (NA) medium. Plates were
incubated at 37 °C for 24 - 48 h. Then, the selected bacteria colonies were streaked on a
Brain Heart Infusion (BHI) medium for antibacterial activities determination.
In the identification of isolated bacteria species, standard taxonomic descriptions
from Sneath [10] were used. Also, API 50CH (API Laboratory Products Ltd.,
Biomerieux) was used to determine the biochemical and carbohydrate fermentation
patterns. The experiment was performed at 37 °C. The results of biochemical tests and
carbohydrate fermentation were determined after 24 and 48 h, and Bacillus species were
identified.