This review examines handling and processing of spleen biopsies and splenectomy specimens with the aim of
providing the pathologist with guidance in optimizing examination and diagnosis of splenic disorders. It also offers
recommendations as to relevant reporting factors in gross examination, which may guide diagnostic workup.
The role of splenic needle biopsies is discussed. The International Spleen Consortium is a group dedicated to promoting
education and research on the anatomy, physiology, and pathology of the spleen. In keeping with these
goals, we have undertaken to provide guidelines for gross examination, sectioning, and sampling of spleen tissue
to optimize diagnosis (Burke [1]). The pathology of the spleen may be complicated in routine practice due to a
number of factors. Among these are lack of familiarity with lesions, complex histopathology, mimicry within several
types of lesions, and overall rarity. To optimize diagnosis, appropriate handling and processing of splenic tissue
are crucial. The importance of complete and accurate clinical history cannot be overstated. In many cases,
significant clinical history such as previous lymphoproliferative disorders, hematologic disorders, trauma, etc,
can provide important information to guide the evaluation of spleen specimens. Clinical information helps
plan for appropriate processing of the spleen specimen. The pathologist should encourage surgical colleagues,
who typically provide the specimens, to include as much clinical information as possible.