Cells were re-suspended in same volume of TE buffer to which 30 mL 10% sodium dodecyl sulfate and 3 mL Proteinase K solution (20 mg/mL in TE) were added followed by incubation at 378C for 1 hour. NaCl (2 mL; 5 M) was added followed by mixing for 30 min,
Cells were re-suspended in same volume of TE buffer to which 30 mL 10% sodium dodecyl sulfate and 3 mL Proteinase K solution (20 mg/mL in TE) were added followed by incubation at 378C for 1 hour. NaCl (2 mL; 5 M) was added followed by mixing for 30 min,