was carried out by mixing the berries with water, and 1 ml was filtered through a Sep-pak C18 column to avoid the interference of sugars, while polyphenols were eluted with MeOH. After washing the column, first Folin–Ciocalteu reagent (1 ml) was added to the column eluate and then, after 5 min, sodium carbonate (10% w/v). Catechin was used as the reference standard. After 90 min, phenols were read at 700 nm. Total anthocyanins were determined by filtering the previous extract using the same Sep-pak C18 column and reading at 520 nm All chemical data are expressed on a dry weight basis to avoid the interference of water loss on data discussion.