A series of experiments with different initial cell concentrations were performed. The particle size and moisture content
were 0.5–1.5 mm and 75%, respectively. No reducing agent was
added. The inoculum culture was concentrated in 0.9% NaCl
aseptically. Serial dilutions of this concentrated cell suspension were used to inoculate the flasks to yield initial cell
concentrations of 2, 4, 10 × 106 cells/g raw sorghum. Samples
were withdrawn from each flask every 6 h.