it was cooled at room
temperature. The lyophilized ampoule of L. helveticus was
broken aseptically in laminar air flow and small amount
(one loop full) of the dried culture was transferred into
tube containing MRS broth. It was mixed properly using
vortex shaker and then incubated at 30°C in an incubator
for 24 to 48 hours. After 48 hours the tubes were observed
for the growth and purity of culture was tested, other
inoculums were prepared by using equal amount of