In order to improve the system, dialysis membranes were replaced by Teflon membranes. In this way, there was not interference by ascorbic acid but response times were longer, due to the slow diffusion of methanol through the membrane, the noise became larger, and the electrode surface became rough after 1 day of continuous use, thus,requiring frequent changing. Despite these inconveniences, the authors demonstrated that plant tissue-based biosensors tolerate the absorption of high molecular weight substances.