Previous studies have established the need for appropriate diluents for cool storage of sperm [5,
8]. Moreover, storage of sperm in diluents with optimal constituents prolongs their lives compared to
undiluted sperm. Morisawa and Suzuki [9] determined the factors whereby the motility of teleost
spermatozoa is initiated, i.e. the decrease or increase in osmolality of the environment. The
composition of the diluent as well as its osmolality also has a significant influence on the success of
storage. Mongkonpanya et al. [3] concluded that a suitable extender should have an osmolality level
near the range of that of fresh sperm. The finding seems to agree with the data in this study, i.e. the
HBSS osmolality value of 233 mOsmo/kg was almost the same as that of the fresh sperm (206
mOsmo/kg), but quite different from those of NaCl and Kurokura (270 and 297 mOsmo/kg
respectively).
Since HBSS extender at all volumes (0.5, 1, 2 and 4 ml) gave the best result from the first part
of the study (Figure 3), it was used to check the motility grade of MGC sperm in different levels of
DMSO (a cryoprotectant usually used in sperm cryopreservation).
Previous studies have established the need for appropriate diluents for cool storage of sperm [5,8]. Moreover, storage of sperm in diluents with optimal constituents prolongs their lives compared toundiluted sperm. Morisawa and Suzuki [9] determined the factors whereby the motility of teleostspermatozoa is initiated, i.e. the decrease or increase in osmolality of the environment. Thecomposition of the diluent as well as its osmolality also has a significant influence on the success ofstorage. Mongkonpanya et al. [3] concluded that a suitable extender should have an osmolality levelnear the range of that of fresh sperm. The finding seems to agree with the data in this study, i.e. theHBSS osmolality value of 233 mOsmo/kg was almost the same as that of the fresh sperm (206mOsmo/kg), but quite different from those of NaCl and Kurokura (270 and 297 mOsmo/kgrespectively).Since HBSS extender at all volumes (0.5, 1, 2 and 4 ml) gave the best result from the first partof the study (Figure 3), it was used to check the motility grade of MGC sperm in different levels ofDMSO (a cryoprotectant usually used in sperm cryopreservation).
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