Another method is to prepare thick vibratome sections of tissue of 35 to
100 μm in thickness with thinner alternating sections for histology and/or
immunocytochemistry.
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The thick vibratome sections can be flat-embedded
under Mylar film, on glass slides, or under plastic coverslips to enable large
areas to be analyzed for apoptosis or for precise selection of architectonic
areas. These plastic-embedded sections and their adjacent sections stained
for light microscopy can help direct precise dissection of small blocks 0.5 to
1 mm large for re-embedding.