The crude extract was filtered and dialyzed by using Sigma (USA)
dialysis membrane-500 (average flat width: 24.26 mm; average
diameter: 14.3 mm; approximate capacity: 1.61 mL/cm) against Dglucose
to remove excess water. Then, the supernatant obtained and it
was lyophilized (Free Zone® Freeze Dry Systems, Labconco, USA) for
further analysis.