Here use of asparagine, CH and
proline were found to be effective for producing high amount of somatic embryo.
Among the three different additives L-aspergine was found most suitable for induction
of somatic embryos from immature embryo derived calli. Requirement of exogenous
supply of amino acids for in vitro somatic embryogenesis has been reported in a number
of plant species, which is also influenced by exogenous supply in culture medium (Basu
et al., 1989; Claparols et al., 1993). In the present study too, it was found that
exogenous application of a specific amino acid, especially asparagine, promoted the
process of somatic embryogenesis and regeneration. Important amino acids were found
to be present in regenerated shoots as well as in embryogenic cultures.
Here use of asparagine, CH and proline were found to be effective for producing high amount of somatic embryo. Among the three different additives L-aspergine was found most suitable for induction of somatic embryos from immature embryo derived calli. Requirement of exogenous supply of amino acids for in vitro somatic embryogenesis has been reported in a number of plant species, which is also influenced by exogenous supply in culture medium (Basu et al., 1989; Claparols et al., 1993). In the present study too, it was found that exogenous application of a specific amino acid, especially asparagine, promoted the process of somatic embryogenesis and regeneration. Important amino acids were found to be present in regenerated shoots as well as in embryogenic cultures.
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