The objective of the experiment was to examine the effect of
herbal-based bio conditioners (AquaHerb) on tiger grouper
against V. parahaemolyticus infection. Challenge tests were
performed with Vibrio parahaemolyticus at a density of 105
cells/ml after three different period of herbal immersion,
namely: 6h, 12 h and 24 h. completely randomized design are
performed with three repetitions and without water exchange
during the therapy. The concentration of AquaHerb are equal
to all period, namely: 1 gr/20 L. Marine water for AquaHerb
administration are treated with mechanical, biological and UV
filter prior to use to eliminate the number of bacteria. Normal
feeding regime and water exchange was conducted after
medication and during observation. During the observation,
water quality was checked regularly with moderately-high