Murashige and Skoog (MS) basal medium
(Murashige and Skoog, 1962) was used for
shoot induction and elongation, and half
strength MS basal medium was used for in
vitro rooting. All media were supplemented
with 3.0% (w/v) sucrose and 0.7% (w/v)
agar. The pH of the medium was adjusted to
5.8 prior to autoclaving at 121°C for 20 min.
The cultures were incubated for a 16/8 h
(light/dark) photoperiod at 25±2 °C
under a fluorescent light.