2.3. In vivo experiments
For the in vivo experiments, a total of thirty 17–20 week-old
male SHR were used. They were divided into two groups of fifteen
animals, that were respectively administered by gastric intubation
1 ml distilled water or 300 mg/kg CFP dissolved in the same
volume of distilled water. Four hours after these administrations,
five of the animals in each group were intraperitoneally administered
1 ml saline. The remaining rats in both groups were divided
into another two groups of five animals that were respectively
administered, by the same procedure, 30 mg/kg L-NAME or 5 mg/
kg indomethacin, both dissolved in 1 ml of saline. Systolic blood
pressure (SBP) was recorded in the different rats by the tail cuff
method [9] before the initial oral administration and also 6 h after.
All the above-mentioned experiments were performed as
authorized for scientific research (European Directive 86/609/CEE
and Royal Decree 223/1988 of the Spanish Ministry of Agriculture,
Fisheries and Food).