PCR amplification of the receiver plasmids was carried out from 5.0 to 10.0 ng (normal protocol) or 0.5 ng (optimized protocol) of template DNA in a 50 μL reaction volume.
PCRamplification of the receiver plasmids was carried out from 5.0 to 10.0 ng (normal protocol) or0.5 ng (optimized protocol) of template DNA in a 50 μL reaction volume.