Actinomycete suspensions were prepared. Tween-80 solution (0.05%) was used to recover the spores, which were adjusted to 106CFU ml−1by the same solution using the Thomas cell. A pathogen suspension was prepared by growing R. solani in PDA dishes at 25◦C for 10 days. Sporeswere directly recovered in sterile distilled water and adjusted to 105CFU ml−1 in the same way.