Reaction mix consisted of 990 ml of emulsified substrate and 10 ml of enzyme solution (50 mg protein). Reactions were carried out at 37 C in a spectrophotometer (UVmini-1240 Shimadzu) as described above.
Reaction mix consisted of990 ml of emulsified substrate and 10 ml of enzyme solution (50 mgprotein). Reactions were carried out at 37 C in a spectrophotometer(UVmini-1240 Shimadzu) as described above.