The threshold
cycle values are determined by the cycle at which the
fluoresecent emission rises above the threshold (Guiletti
et al., 2001). Because SYBR Green is not sequence specific,
melting curves can be used to analyze different products.
With this method primer, dimers can be distinguished
by their lower melting temperature (Tm) (Ririe et al.,
1997; Wittwer et al., 2001). The Ct (cycle at which the
amplification crosses a specified threshold) decreases as
the amount of target increases (Guiletti et al., 2001