An extremely low acid (ELA) pretreatment using 0.06% (w/w) sulfuric acid at 170 C for 15 min was
employed to extract non-glucan components from Saccharina japonica, a brown macroalgae. Subsequent
simultaneous saccharification and fermentation (SSF) was conducted using Saccharomyces cerevisiae DK
410362 and cellulase (15 FPU/g-glucan) and ß-glucosidase (70 pNPGU/g-glucan). Deionized water was
used for making fermentation suspension. After the ELA pretreatment, a glucan content of 29.10% and
an enzymatic digestibility of 83.96% was obtained for pretreated S. japonica. These values are 4.2- and
2.4-fold higher, respectively, than those of obtained with untreated S. japonica. In SSF, a bioethanol concentration
of 6.65 g/L was obtained, corresponding to a glucose equivalent concentration of 13.01 g/L,
which indicated an SSF yield of 67.41% based on the total available glucan of the pretreated S. japonica.
The remaining separated liquid hydrolysate, which contains mannitol and alginate-derived oligosaccharides
can be applied to other fermentations.
An extremely low acid (ELA) pretreatment using 0.06% (w/w) sulfuric acid at 170 C for 15 min wasemployed to extract non-glucan components from Saccharina japonica, a brown macroalgae. Subsequentsimultaneous saccharification and fermentation (SSF) was conducted using Saccharomyces cerevisiae DK410362 and cellulase (15 FPU/g-glucan) and ß-glucosidase (70 pNPGU/g-glucan). Deionized water wasused for making fermentation suspension. After the ELA pretreatment, a glucan content of 29.10% andan enzymatic digestibility of 83.96% was obtained for pretreated S. japonica. These values are 4.2- and2.4-fold higher, respectively, than those of obtained with untreated S. japonica. In SSF, a bioethanol concentrationof 6.65 g/L was obtained, corresponding to a glucose equivalent concentration of 13.01 g/L,which indicated an SSF yield of 67.41% based on the total available glucan of the pretreated S. japonica.The remaining separated liquid hydrolysate, which contains mannitol and alginate-derived oligosaccharidescan be applied to other fermentations.
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