Standards of a-tocopherol in ethanol were used to determine the molar absorption coefficient, the concentration range for linearity and validity of the Beer’s law, and the precision of the method, by measuring the absorbance at 695 nm (A695). Spectrophotometric measurements were performed on three different occasions at several-day intervals on seven analyte groups whose A695 varied within the range 0.15–0.85. Repeatability (within-run precision) and reproducibility (betweenrun precision) were estimated by regression analysis, and they were expressed as relative standard deviation (RSD, %) of the slope (molar absorption coefficient) of A695 vs molar concentration curves. The mean molar absorption coefficient obtained from the overall linear regression relating A695 vs concentration of 21 values at the 95% confidence level was e 5 (4.0 6 0.3) 3 103 M21 cm21 with an RSD 5 1.3, 2.2, and 2.4% in each case and a r2 = 0.997 (reproducibility). Linearity was observed between 2 3 1025 and 2 3 1024 M a-tocopherol.