Nevertheless, as it has been demonstrated in various studies, ethylene bridged silicabased RP columns can be used at temperatures above 100 C without
the fear of a rapid breakdown
The newest generations of polydentate silica phases have been claimed for separations up to 200 C,
but experimental results indicate that phase stability is still far from being satisfactory (García-Lavandeira et al., 2011).
In practice, the stationary phases with the highest temperature stability are based on materials other than silica,
e.g. graphitized carbon, zirconium oxide-based and polystyrene/
divinylbenzene phases. Such columns are stable at temperatures
of 150 C or higher provided that any PEEK present in the column
hardware is replaced by stainless steel.