3.2 Adherence and yield of cells
The maximum cell adherence was observed in culture plates using BM III (M 199) and its tissue extracts (Table 2). Cell adherence and a short pseudopodia network were also observed in the plates using BM I (L-15) and its tissue extracts. The pseudopodial network was not observed in cultures using BM II (F12) and with tissue extracts except a lone occurrence of pseudopodial network in a plate with BM II with ME. Yield of cells was more in BM I and less with BM II without any tissue extracts. However cultures with BM II liberated more cells when incorporated with tissue extracts (Table 2). Statistically there was no significant difference between treatments with different basal media. Significant difference in cell yield was observed between treatments incorporated with tissue extracts. Maximum significant difference was observed between treatments with mantle extract and basal medium and next between treatments with whole body and basal medium in all three media (L-15, F12 and M199) compositions.
Table 2. Cell yield (mean ± SD) and cell adherence in media L15, F12 and M199 with tissue extracts