Blotter test According to Wang and Tylkowska (2003)
and Mew and Misra (1994), three layers of 9.0 cmdiameter
white blotter papers soaked in sterile distilled
water were placed in petri plates allowing NUV light to
penetrate. Twenty-five wheat seeds were placed in
each plate and four plates of seeds per germplasm were
tested. The seeds were incubated for 7-10 d at 22°C
with alternate 12 h periods of NUV light and darkness.
The plates were approximately 40 cm below the lights