3.2. Acute toxicity study
Male mice exposed to a single dose (5–1500 mg/kg b.w.) of C. citratus EO presented no alterations in general behavior when compared to the controls. There was only one death in the group treated with lemongrass EO at 2000 mg/kg dose. The animals that received doses higher than 3000 mg/kg b.w. showed abnormalities such as torpor and cyanosis, while those exposed to 4000 mg/kg b.w. died within 24 h after the oil administration. Therefore, the lethal dose, calculated by the probit test, was around 3500 mg/kg.