3.1. Hydrolysis of phytate during the breadmaking process
Preliminarily, washed cells of S. cerevisiae LC3 were directly assayed to determine the ability to hydrolyse phytate during fermentation, since the only enzymes that may come in contact with phytate in the dough are secreted or cell-bound. Compared to cellular crude protein extract, washed cells of S. cerevisiae LC3 showed similar (P ¼ 0.352) enzymatic activity on phytate. S. cerevisiae LC3 was used as starter together with L. plantarum LP41 to ferment whole-meal flour