A 1.0 mL aliquot of a 0.5 mmol/L DPPH in methanol (dissolved in 50% methanol solution) and 4.0 mL of the appropriately diluted sample were vigorously vortexed in a test tube with cap tube.
A 1.0 mL aliquot of a 0.5 mmol/L DPPH in methanol (dissolved in 50% methanolsolution) and 4.0 mL of the appropriately diluted sample were vigorouslyvortexed in a test tube with cap tube.