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The kinetics of elimination of the [PSI+] prion by growth in 3 mM GdnHCl. For each of the four different strains indicated, cells were allowed to undergo up to 15 generations of continual growth in YEPD supplemented with 3 mM GdnHCl. The proportion of cells that gave rise to [PSI+] colonies was plotted against the number of generations of growth. The arrows indicate the first appearance of prion-free ([psi−]) cells