Microorganisms and cultures:
Spirogyra: The alga Spirogyra species is multicellular, filamentous and microscopic in nature. Spirogyra was
collected from Mir Alam Lake-situated in Hyderabad Metropolis-Andhra Pradesh, India. The algal mat was
collected in sterile containers and transferred to the laboratory.
Two fungal cultures- Aspergillus niger MTCC2196 (for saccharification) and Saccharomyces cerevisiae MTCC
170 for (fermentation) were procured from MTCC (Microbial Type Culture Collection Centre and Gene Bank)
Chandigarh, India. The fungi Aspergillus niger was cultured and maintained on potato dextrose agar medium at
300C. The yeast Saccharomyces cerevisiae was cultured and maintained on YPD (Yeast extract, peptone and
dextrose) agar media at 300C.