This research aims to: 1) to develop an e-Learning of Computer Assisted Instruction on a needs analysis. Facts and tracing. 2) to compare the learning outcomes between Pre-test and Post-test of Computer Assisted Instruction on a needs analysis. Facts and tracing. 3) to assess the satisfaction of students after using e-Learning of Computer Assisted Instruction on a needs analysis. Facts and tracing. The instrument used for this research to: 1) to develop an e-Learning of Computer Assisted Instruction on a needs analysis. Facts and tracing. 2) Quiz achievement of four multiple-choice selection of 25 items. 3) A satisfaction rating in the e-Learning of Computer Assisted Instruction on a needs analysis. Facts and tracing. The sample group of this research. This study used simple random sampling for 30 sample from the 4th year students of computer education program in Nakhon Si Thammarat Rajabhat. The statistics used for data analysis were percentage, mean, standard deviation and t-test for dependent.