was used with some modification. A 72 h old
bacterial culture was centrifuged at 8000 for 15 min at
4 C and the supernatant was used for Pi quantification.
According to the method 200 ll supernatant was taken in
a 50 ml volumetric flask, and then 10 ml of chloromolybdic
acid was added to it and made up volume with
sterile distilled water up to 40 ml. Then 1 ml of chlorostanous
acid was added and after mixing made the
volume up to 50 ml very quickly with sterile distilled
water. The amount of phosphate solubilized by the bacterial
cells was calculated using the standard curve of
different concentration of 10 ppm stock solution of
KH2PO4.