Statistical analyses were done using SPSS programme (SPSS Inc., Chicago, IL, USA). The normality of data distribution was checked using the Kolmogorov–Smirnov test.
One-way ANOVA was performed to examine differences among groups.
The significance of mean differences between groups were tested by Duncan. Values were given asmean±standard error. Only egg internal and external quality characteristics were compared with ANCOVA (difference and repeated contrast method) and values were given as estimated marginal means ± standard error of mean with adjusting egg weight.
Level of significance was taken as P < 0.05.