Methods
Butabushi preparation
For Pork and Beef,connective tissue and excess fat were removed.
All samples were then steamed in Combi-Oven for 30 min at 100 C. Following cooking,samples were
transferred to a smoker,and smoked for 6 h with hickory and apple chips. Afterwards samples were placed in dehydrator on racks for 8 h at 40 C. Both inoculated and un-inoculated pork were placed on raw sushi rice in foil-lined containers and sealed.Inoculum used were both fresh and freeze dried isolates of mold native to our environment,as well as A. orzyae. Fungal isolates were made into separate slurries with warm water,and brushed