2.4. Statistical analysis
Data from each treatment were subjected to one-way
analysis of variance performed with the Statistical Package
for Social Sciences Windows version, release 7.5.1
(SPSS Inc., Chicago, Illinois, USA). When overall differences
were significant (P<0.05), Scheffé's Multiple
Range Test was used to compare the means between
individual treatments (Zar, 1984). All percentage data
were arcsine-transformed before analysis. Broken-line
model (Robbins et al., 1979) was used to estimate the
maximum feed restriction which does not hamper
shrimp growth. The equation used in the model was as
follows: y=L−U(R−x), where y is the value of the
parameter, L is the maximum value of the parameter,
U is the slope, x is the level of feed restriction and R is
the optimum value.
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