Absorbance at 280, 545, 565, 620 and 650 nm
and absorption spectra were recorded on a
Perkin–Elmer (Beaconsfield, UK) Lambda-16
UV-Visible spectrophotometer with a 1 cm light
path. Fluorescence emission spectra were recorded
on a Shimadzu (Kyoto, Japan) RF5001 spectrofluorometer.
For steady state polarized fluorescence
measurements the spectrofluorometer was