The overall prevalence of bacterial species in 100 mastitic milk samples of buffaloes during present
investigation is shown in Table 1. Of the samples collected and examined on various culture media, 70
(70%) were found positive for bacterial growth, while 30 (30%) were shown no growth on culture media
and considered to be free from any bacterial infection. It is concluded from the present study that majority
of the mastitis cases in buffaloes are caused by bacterial species. However, mastitic milk samples which
exhibited no growth on culture media could be attributed to some predisposing factors of non-bacterial
etiology or buffaloes may be treated previously with a variety of antibiotics prior to sampling.
During present survey on clinical mastitic milk samples of buffaloes, 70 mastitic milk samples were
positive for bacterial infections, of which 29 (41.42%) were purely caused by Staphylococcus aureus,
whereas 5 (7.14%) samples were recorded as positive for Staphylococcus aureus and other species,
while remaining 36 (51.43%) were also found positive other than Staphylococcus aureus (Table 2). The
study reveals that the Staphylococcus aureus was the common bacterial species responsible to cause
clinical mastitis in buffaloes.