The sugar content of the apple pomaces ranged between 36.6 g/L for the first year and 42.6 for the second, with similar ethanol yields at the end of alcoholic fermentation in all cases, presenting mean values of 2.8% (v/w) (Fig. 1). Microbiological analysis showed yeast concentrations above 106 cfu/ml and a degree of implantation of the inoculated strains equal to or higher than 90% in all cases (data not shown). Although all the strains showed similar fermentation kinetics (Fig. 1), it is worth to note that the fermentation with Levuline and the enzyme took place before 14 days, which could be relevant from the industrial perspective. Moreover, it was observed that under the conditions employed in our study, in which ethanol production is not high, the H.u. 283 strain was able to complete the consumption of sugars, with a production of ethanol equivalent to those detected in the experimental units fermented with Saccharomyces strains. On the other hand, the concentrations of lactic and acetic bacteria were lower than 103 cfu/ml in all cases.