GI50 is the concentration of the anti-cancer drug that inhibits the growth of cancer cells by 50%. In other words, after giving the drug, there is a 50% reduction in cancer cell proliferation. IC50 is the concentration of a drug that inhibits a biological activity by 50%. Besides GI50 and IC50, TGI also be one of parameters which is the concentration of test drug and it also signifies cytostatic effect. Differ from TGI; LC50 is Lethal Concentration 50, which signifies cytotoxic effect. In other words, LC50 is the concentration in water having 50% chance of causing death to aquatic life