Several technical artefacts were considered as possible
causes for the heterozygote deficiency, including incorrect
scoring of adjacent alleles due to stuttering at dinucleotide
repeats, missing larger alleles due to inefficient amplification,
or complications due to a high number of alleles
relative to the sample size. None of these, however, had any
effect on the amount of heterozygote deficiency (analysis
not shown). Heterozygote excesses were observed only at
Pse343 (11 of 14 samples and overall), Pse36 from West
Delta, LA and PseC48 and Pse101 from Mexico, but none
of these deviations was statistically significant.