The cumulus-free oocytes were stained with 10 g/mL of propidium iodide (Sigma) in PBS containing 0.1% of polyvinyl alcohol (Sigma) and incubated for 15 min in darkness.
The cumulus-free oocytes were stained with 10g/mL of propidium iodide (Sigma) in PBS containing0.1% of polyvinyl alcohol (Sigma) and incubated for 15min in darkness.